Tetracycline-Free Fetal Bovine Serum: Premium Cell Culture Supplement

Astor Scientific offers a Fetal Bovine Serum, Tetracycline Free (AS-F0500) that is specially processed and tested to ensure compatibility with tetracycline-controlled gene expression systems. This product provides a reliable serum source free of residual tetracycline compounds.

Key Features & Benefits

• Tetracycline-Free Assurance: This FBS is functionally tested to confirm it contains no detectable levels of tetracycline, chlortetracycline, or oxytetracycline, using sensitive LC-ESI-MS/MS methods with detection limits < 0.05 mg/L. 

• Optimized for Tet Systems: It is tailored for use with tetracycline (Tet)-based gene expression systems. Because trace tetracyclines can interfere with the regulation of TRE (tetracycline response element) promoters, this serum ensures maximal induction ranges and consistent expression control.

• Storage Conditions: Must be stored at –20 °C to maintain stability and integrity.

• Applications & Usage Context

  This specialized FBS is primarily used in molecular biology and cell culture settings where precise control of gene expression is necessary, especially when using Tet-inducible systems. Applications include:

  • Transgenic or inducible expression studies

  • Gene regulation and promoter analysis

  • Controlled knockdown or overexpression experiments

  • Cell lines engineered with Tet-on or Tet-off constructs

  Because standard FBS may contain trace antibiotic residues, using a tetracycline-free variant is critical in systems where even small contaminations can skew baseline gene expression levels or induction dynamics.

  Considerations & Limitations

  • Research Use Only: As with most cell culture reagents, this FBS is intended for research purposes and not for clinical or therapeutic use.

  • Batch Variability: While the product is tested, inherent biological variability in serum lots means you may need to validate performance (e.g. induction fold, background leakiness) batch to batch.

  • Cost & Supply: The price is relatively high, and the product may frequently be out of stock, so planning ahead is important.

  • Cold Chain Handling: Proper freezing and cold shipment are crucial to prevent degradation or spoilage.

  How to buy tetracycline free FBS

  To acquire this reagent, researchers can visit the product page for Fetal Bovine Serum, Tetracycline Free (AS-F0500) on the Astor Scientific website. The page lists available variants (if in stock), pricing, and ordering details.